RNA Methodologies A Laboratory Guide For Isolation And Characterization

The isolation of biologically competent and chemically stable RNA is central to molecular biology. There remains an academic imperative to unify the many complex facets of the characterizations of cellular biochemistry with particular regards to the use of RNA as a parameter of gene expression. This laboratory guide is a growing collection of tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA. The focus herein is how these methodologies can be used in the study of transcriptional- and post-transcriptional regulation of gene expression. KEY FEATURES: *New sections describing RNA isolation, subtraction-suppression PCR, high-throughput methods, bioinformatics, and RNAi *Explains proper handling, storage, and manipulation of RNA *An expanded section on RT-PCR, RACE, and related techniques *Trouble-shooting options are incorporated throughout the textFarrell, Robert E., Jr. is the author of 'RNA Methodologies A Laboratory Guide For Isolation And Characterization', published 2005 under ISBN 9780122496967 and ISBN 0122496965.